Prime EP-3200r Driver
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Prime EP-3200r Driver
DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation Prime EP-3200r Artificial plant minichromosomes comprising a functional centromere which specifically bind centromeric protein C CENPC and methods for making such minichromosomes are Prime EP-3200r.
When a transgene is Prime EP-3200r into a plant genome, it is usually in a random fashion and in an unpredictable copy number. Accordingly, research efforts have been directed toward better controlling transgene integration.
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Given this need, researchers have wondered if the answer might lie in the use of artificial minichromosomes. These are man-made linear or circular DNA molecules constructed from cis-acting DNA sequence elements that provide replication and partitioning of the Prime EP-3200r minichromosomes.
It is believed that production of artificial chromosomes would reduce or eliminate some issues associated with random genomic integrations into a native plant chromosome, for example linkage drag due to association of the transgene with genomic material from Prime EP-3200r host plant. Isolated centromeres from unicellular organisms, such as yeast, do not function in higher eukaryotes.
Patent Prime EP-3200r, issued to Richards et al.
Patent 7,, issued to Luo et al. Patent 7,, issued to Richards et al. Prime EP-3200r 7,, issued to Copenhaver et al.
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In a second embodiment, an artificial plant minichromosome of the invention comprises Prime EP-3200r retrotransposable element selected from the group consisting of CentA, CRM1, and CRM2. In a third embodiment, the artificial plant minichromosome of the invention Prime EP-3200r comprises at least one functional telomere.
In a fourth embodiment, Prime EP-3200r functional centromere, comprised by the artificial plant minichromosome specifically binds centromeric protein C CENPC. In a fifth embodiment, a corn plant can comprise any of the artificial minichromosomes of the invention. In a sixth embodiment, the present invention concerns an artificial plant minichromosome comprising a functional centromere, wherein the centromere specifically binds centromeric Prime EP-3200r C CENPC.
In a seventh embodiment, the invention concerns an isolated polynucleotide Prime EP-3200r In an eighth embodiment, the isolated polynucleotide of the invention comprises a retrotransposable element which is selected from the group consisting of CentA, Prime EP-3200r, and CRM2. In a ninth embodiment, the invention concerns an isolated polynucleotide comprising: In a tenth embodiment, the invention concerns an isolated polynucleotide comprising: In an eleventh embodiment, the invention concerns a recombinant construct comprising any of the isolated polynucleotides of the invention as well as a transgenic corn plant comprising such recombinant constructs.
In a twelfth embodiment, the invention concerns a Prime EP-3200r for making a transgenic corn Prime EP-3200r comprising an artificial plant minichromosome having a functional centromere the method comprising: The mixture can also comprise a polynucleotide encoding a polypeptide for stimulating cell growth wherein the polypeptide is selected from the group consisting of a wuschel, a baby boom, a RepA, or a Lec1.
Copies of this patent or patent application Prime EP-3200r with color drawing s will be provided by the Office upon request and payment of the necessary fee. The invention can be more fully understood from the Prime EP-3200r detailed description and the accompanying drawings and Sequence Listing, which form a part of this application. Calli were derived from immature embryos transformed with linearized BAC clone pool 1 retrofitted with Tn Prometaphase left and metaphase right Prime EP-3200r both show the 20 native chromosomes plus 1 minichromosome arrows and insets.
Both minichromosomes are positive for the CentC green - color; white - greyscale centromere-specific repeat and the unique marker probe red - color; white - greyscale specific Prime EP-3200r the transformation construct, with both CentC and being essentially colocalized in the minichromosome insets. Panel A shows a metaphase nucleus showing the 20 native chromosomes plus 2 minichromosomes box.
Both minichromosomes are positive for the CentC green - color; white - greyscale centromere-specific repeat and the unique marker probe red Prime EP-3200r color; white - greyscale specific to the transformation construct.